You Super Mario thugs is in the wrong room

from Eminem (Ft. Slaughterhouse & Yelawolf) – BET Shady 2.0 Cypher Lyrics on Rap Genius

Meaning

In the video game Super Mario 64, Mario would go to different rooms for different levels of the game in order to find Princess Peach but he did not know which room she was in. So like Mario, these thugs are in the wrong room!

May also refer to Super Mario Bros., where upon beating each level, Toad would tell you:

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